Divergent Regulation of CBF Regulon on Cold Tolerance and Plant Phenotype in Cassava Overexpressing Arabidopsis CBF3 Gene

Cassava is a tropical origin plant that is sensitive to chilling stress. In order to understand the CBF cold response pathway, a well-recognized regulatory mechanism in temperate plants, in cassava, overexpression of an Arabidopsis CBF3 gene is studied. This gene renders cassava increasingly tolerant to cold and drought stresses but is associated with retarded plant growth, leaf curling, reduced storage root yield, and reduced anthocyanin accumulation in a transcript abundance-dependent manner. Physiological analysis revealed that the transgenic cassava increased proline accumulation, reduced malondialdehyde production, and electrolyte leakage under cold stress. These transgenic lines also showed high relative water content when faced with drought. The expression of partial CBF-targeted genes in response to cold displayed temporal and spatial variations in the wild-type and transgenic plants: highly inducible in leaves and less altered in apical buds. In addition, anthocyanin accumulation was inhibited by downregulating the expression of genes involved in its biosynthesis and by interplaying between the CBF3 and the endogenous transcription factors. Thus, the heterologous CBF3 modulates the expression of stress-related genes and carries out a series of physiological adjustments under stressful conditions, showing a varied regulation pattern of CBF regulon from that of cassava CBFs.

Introduction

Cassava (Manihot esculenta Crantz) is widely cultivated for its starchy storage roots in tropics and subtropics where it provides a substantial source of staple food and animal feed (Cock, 1982; Hillocks et al., 2002). In China and other Southeast Asian countries, cassava is mainly used for starch, modified starch, and bioethanol production (Nguyen et al., 2007; Howeler, 2015). As a native to a warm habitat of tropics, cassava is adapted to the zone within a latitude 30° north and south of the equator and is thus, categorized as an extremely chilling-sensitive species (Alves, 2002; Huang et al., 2005). Therefore, low temperature is one of the major limiting factors for its geographical distribution and productivity, restricting its growth and survival below 18°C (An et al., 2012). With the cassava industrialization in China, expanding its cultivation has been promoted in subtropical regions, especially up to latitude 30°N (Jansson et al., 2009). Encountering the low temperature during late autumn, winter, and early spring, sometimes accompanied with extreme frozen climate is a major obstacle to high cassava yield and stem storage for propagation (An et al., 2012). Therefore, to acquire a prolonged growth period (i.e., early planting and late harvesting) in the high-latitude regions and a stable yield under stressful conditions, novel cassava cultivars with improved abiotic stress tolerance are in demand (Liu et al., 2011; Xu et al., 2014).

In order to cope with abiotic stresses that adversely affect the growth and yield, plants have evolved a set of adaptation mechanisms, including physiological and molecular processes (Bohnert et al., 1995; Ahuja et al., 2010). Expression of some stress-induced genes that might be involved in stress tolerance, transcription regulation, or signal transduction has been extensively studied in plants such as Arabidopsis and rice (Thomashow, 1999; Shinozaki et al., 2003; Chinnusamy et al., 2007; Nakashima et al., 2009). During transcriptional regulation, transcription factors (TFs) respond to stress stimuli via regulation of the downstream cascade of the target genes to protect the plant cells from injury. Among abiotic stress-regulated TFs, a small family of transcriptional activators known as dehydration-responsive element binding factors 1 (DREB1s)/C-repeat-binding factors (CBFs), including CBF1, CBF2, and CBF3 (also known as DREB1B, DREB1C, and DREB1A, respectively), were identified as the central regulators (Mizoi et al., 2012; Zhao et al., 2016). These factors bind to the low-temperature responsive DNA regulatory element termed C-repeat (CRT)/dehydration response element (DRE) of promoters in cold-inducible genes (Yamaguchi-Shinozaki and Shinozaki, 1994; Gilmour et al., 1998; Thomashow, 1999; Thomashow et al., 2001). In Arabidopsis, CBFs are essential for cold acclimation and freeze tolerance, which is controlled by regulating the expression of approximately 12% of the cold-responsive (COR) genes (Fowler and Thomashow, 2002) out of the recently confirmed 414 COR genes using CRISPR/Cas9 based CBF mutants (Zhao et al., 2016). Moreover, the constitutive expression of CBF genes induces the accumulation of mRNAs from genes that contain the CRT/DRE motif in their promoters, the CBF regulon, as well as from those which exhibit an increase in freezing and other abiotic stresses tolerance (Kasuga et al., 1999; Zhao et al., 2016). After being identified in Arabidopsis, many CBF homologs have been found in various plant species, including both dicots and monocots. These homologs have also demonstrated a key role in multiple stress response and tolerance by regulating the corresponding CBF-regulons (Jaglo et al., 2001; Hsieh et al., 2002a,b; Dubouzet et al., 2003; Ito et al., 2006; Chen et al., 2008). These results indicate that the upregulation of CBF expression is a feasible approach in improving multi-stress tolerance of agriculturally critical crops (Mickelbart et al., 2015).

C-repeat-binding factor cold response pathway is activated during cold acclimation of temperate plants, which not only causes metabolic changes including accumulation of soluble sugars and proline but also inhibits the plant cell growth through regulating different regulons (Gilmour et al., 2004; Park et al., 2015; Zhao et al., 2016). In addition to the improved stress tolerance, constitutive expression of CBFs in transgenic Arabidopsis, tomato, wheat, and barley exerts an adverse effect on plant phenotypes, such as growth retardation and yield reduction (Jaglo-Ottosen et al., 1998; Hsieh et al., 2002a,b; Morran et al., 2011). Thus, these phenotypic responses underlie the CBF interplay of the balance of cold stress regulation and plant growth (Zhao et al., 2016). The tropical plants, such as cassava, rice, maize, and tomato, are very sensitive to chilling stress and largely lack the capacity for cold acclimation (Zhu et al., 2007). The CBF orthologous gene MeCBF1 of cassava has been proved to be induced by cold; nevertheless, compared with Arabidopsis CBFs, its retarded expression in response to cold and attenuated CBF-regulon might lead cassava to chilling sensitivity (An et al., Unpublished data). Therefore, understanding the CBF-mediated response to abiotic stresses in the non-cold-acclimating plants such as cassava may reveal the regulatory differences between temperate and tropical plants during evolution.

Herein, we report the improved cold and drought tolerance of transgenic cassava plants overexpressing Arabidopsis CBF3 gene. Apparently, ectopic expression of CBF3 modulates the expression of many stress-related genes, some of them belonging to CBF-regulons, subsequently resulting in increased accumulation of osmolytes and reduced cell damage under normal and stressful conditions. Intriguingly, growth retardation and downward leaf curling in the CBF3 overexpressing cassava indicate an altered CBF regulon that is regulated by the heterologous CBF gene.

Materials and Methods

Plasmid Construction and Cassava Transformation

The full-length CBF3 complementary DNA (cDNA) of Arabidopsis (AtCBF3) was firstly cloned into the pMD18-T vector (TaKaRa Biotechnology, Dalian, China). The released AtCBF3 fragment was then cleaved by restriction endonucleases, KpnI and PstI, and inserted into the multiple cloning sites between CaMV 35S promoter and NOS terminator of a modified pCAMBIA1301 to generate the binary vector pC35S-AtCBF3 (Supplementary Figure 1). The construct was mobilized into Agrobacterium tumefaciens LBA4404, which was used for cassava transformation by friable embryogenic callus-mediated method (Zhang and Gruissem, 2004).

Southern Blotting Analysis of Transgenic Cassava Plants

Genomic DNA was isolated from mature leaves of transgenic and wild-type (WT) cassava plants using the CTAB DNA extraction method. Twenty μg of total DNA was digested overnight with EcoRI. The fragmented product was separated on a 0.8% agarose gel and transferred onto a Hybond-N⁺ membrane (Amersham Biosciences, Piscataway, NJ, USA). The blot was hybridized with the DIG-labeled hygromycin phosphotransferase (HPT) gene probe and exposed to X-ray film for signal detection. The HPT probe was amplified by PCR using the following primers: 5′-TGAAAAAGCCTGAACTCACCG-3′ (forward) and 5′-TATTCTTTGCCCTCGGACG-3′ (reverse). The PCR program was as follows: 3 min at 94°C; 28 cycles of 30 s at 94°C, 30 s at 55°C, and 40 s at 72°C; followed by 10 min at 72°C; and finally stored at 4°C. DNA probe preparation, hybridization, and membrane washing were performed using DIG High Prime DNA Labeling and Detection Starter Kit II according to the manufacturer’s instructions (Roche, Grenzacherstrasse, Basel, Switzerland).

Growth Conditions and Abiotic Stress Treatment of Cassava Plants

One-month-old in vitro seedlings of AtCBF3 overexpressing lines (OE) and WT were planted into soil pots and grown in the greenhouse at 30°C/25°C with 16 h/8 h light/dark cycle and 30–40% relative humidity. For cold stress, 1-month-old plants grown in the greenhouse were exposed to 0°C for 24 h and then recovered for 1 month. For drought stress, stem-propagated pot-grown plants were cultivated in the greenhouse for 3 months under a fully watered regime (approximately 20% water content) and then the water was withdrawn for 21 days until the plants showed distinct symptoms of injury.

For field-grown cassava, about 6-month-old plants were subjected to seasonal changes. At the temperature below 15°C/8°C for day/night in November, the WT plants began to exhibit cold injury. During field growth, six different tissues of transgenic line OE-3 and WT, including apical buds, immature leaves, mature leaves, old leaves, petioles, and young stems, were harvested under three conditions of different day/night temperatures (September: 33°C/25°C as normal temperature; October: 25°C/16°C as cold treatment I, November: 15°C/8°C as cold treatment II), and frozen in liquid nitrogen for RNA extraction. Each treatment had more than three plants per line, and all treatments were conducted in three biological replicates.

Measurements of Proline, Malondialdehyde (MDA), Electrolyte Leakage, and Relative Water Content (RWC)

One-month-old transgenic OE lines and WT plants were divided into two groups (normal growth and cold treatment), each group containing about 12 plants and each independent line consisting of at least three plants. After the plants had been exposed to 4°C for 12 and 24 h, approximately 0.5 g leaf tissue was harvested for each sample and extracted with various reagents according to different assays. Proline concentrations were measured by the sulfosalicylic acid-acid ninhydrin method (Bates et al., 1973). Malondialdehyde (MDA) equivalent was measured as described previously (Hodges et al., 1999). Electrolyte leakage was assessed following the method by Gilmour et al. (2000).

The RWC of cassava plants under normal growth conditions and drought stress was measured according to Gaxiola et al. (2001). The drought tolerance of these plants was evaluated by growing the plants for 3 months under a fully watered regime and then depleting water for about 3 weeks until these plants displayed obvious injury symptoms. The leaves from OE lines and WT were excised and their fresh weights recorded immediately. Their rehydrated weight was determined after floating the leaves in deionized water at 4°C overnight. Finally, they were dried in an oven at 70°C overnight and weighed. Each measurement was repeated three times. The RWC was calculated according to the formula: RWC = (fresh weight – dry weight)/(rehydrated weight – dry weight) × 100%.

Semi-Quantitative and Real-Time RT-PCR Analysis

Total RNA was extracted from leaves of 1-month-old cassava plants grown under normal conditions or cold stress (4°C for 12 days) using TRIzol reagent according to the manufacturer’s protocol (Invitrogen). The RNA quality was determined on an agarose gel by ethidium bromide staining. Reverse transcription was performed using the ReverTra Ace qPCR RT Kit according to the manufacturer’s protocol (TOYOBO, Osaka, Japan). The cDNA was directly used as templates for semi-quantitative RT-PCR or diluted fivefold for real-time RT-PCR amplification on a Bio-Rad CFX96 thermocycler (CFX96 thermocycler (Hercules, CA, USA) with gene-specific primers (Supplementary Table 1). The quantitative variation between different samples was evaluated by the 2^-ΔΔCt method, and cassava β-actin was used as an internal control. All of the samples were measured in triplicate, and the experiments were performed on three biological replicates.

Statistical Analysis

All data were determined from at least three biological replicates and are presented as mean ± SD. Asterisks indicate significant differences compared with the WT at ^∗P < 0.05 or ^∗∗P < 0.01 by Student’s t-test.

Results

Overexpression of Arabidopsis CBF3 Gene in Cassava

Six AtCBF3 overexpressing (OE) transgenic cassava plant lines regenerated from transformed friable embryogenic calli were multiplied by shoot cultures in vitro. Their transgene integration patterns were verified by the DIG-labeled HPT probe using Southern blotting analysis. Four out of six OE lines were identified as single copy insertion into the genome by EcoRI digestion (Figure 1A). Their AtCBF3 transcript level initially analyzed by semi-quantitative RT-PCR confirmed the high expression of the genes in three lines, OE-1, OE-3, and OE-5 (Supplementary Figure 2). OE-3 and OE-5 lines showed a remarkable overexpression of the CBF3 gene, approximately several 1000-fold higher than that of the OE-1 line, by real-time RT-PCR analysis (Figure 1B). Therefore, these three lines together with WT were used for subsequent studies.

FIGURE 1

FIGURE 1. Molecular and phenotypic characterization of Arabidopsis CBF3 overexpressing cassava. (A) Transgene integration pattern in wild-type (WT) and transgenic (OE) lines by Southern analysis. (B) Quantitative analysis of the DREB1A/CBF3 expression in WT and three single-copied transgenic lines (OE-1, OE-3, and OE-5) by real-time RT-PCR. (C) Leaves collected from field-grown plants. (D) Plant status in the field by comparison of WT and OE-3 plants. (E) Plant height and internode length between WT and OE plants. Data are presented as mean ± SD of three independent assays; asterisks indicate significant differences compared with the WT, ^∗P < 0.05 or ^∗∗P < 0.01 by Student’s t-test.

Similar to the most transgenic plant lines overexpressing CBF subfamily members that showed undesired side-effects such as severe growth retardation, the OE cassava lines also displayed downward leaf curling, especially in OE-3 and OE-5 (Figure 1C), and severe retardation under normal growth conditions in the field (Figure 1D). Plant height and internode length of the OE lines were significantly shorter than those of WT (Figure 1E), about 50%. Upon harvest, their phenotypes of storage roots were photographed (Figure 2A). Noticeably, the storage root number and yield of OE-3 and OE-5 were reduced to 30–50% of WT (Figures 2B,C). The OE-1 plants showed less change in these traits. These phenotypic changes were obviously dependent on the transcript abundance of the AtCBF3 gene.

FIGURE 2

FIGURE 2. Characteristics of storage roots harvested from the field-grown wild-type (WT) and transgenic (OE) lines. (A) Storage root phenotypes. (B) Storage root number per plant. (C) Storage root weight per plant. Data are presented as mean ± SD of three independent assays; asterisks indicate significant differences compared with the WT, ^∗P < 0.05 or ^∗∗P < 0.01 by Student’s t-test.

Expression of Arabidopsis CBF3 Enhances Cassava Tolerance to Cold Stress

To investigate whether overexpression of AtCBF3 confers abiotic stress tolerance in cassava, 1-month-old OE and WT seedling plants grown in the greenhouse were exposed to cold stress (0°C) for 24 h, followed by recovery of the growth under standard conditions (25°C). The OE transgenic plants recuperated better than the WT (Figure 3A). In particular, after 1 month, the OE-3 and OE-5 lines continued with a vigorous growth, including sprouting new leaves; while OE-1 plants only survived. Contrastingly, WT plants could not be recovered and finally perished.

FIGURE 3

FIGURE 3. Performance of Arabidopsis CBF3 overexpressing cassava in response to low temperature. (A) Pot-grown plants in the greenhouse before and after cold treatment (0°C, 24 h) for 1 week and 1 month recovery under normal growth condition. (B) Apical shoots, leaves, and apical buds harvested from WT and OE transgenic plants in the field under chilling condition in early November.

In the field, under the seasonal transition from autumn to early winter, the phenotypic exhibition of these plants was also different. When the temperature decreased to 15°C/8°C (day/night) at the beginning of November, WT plants displayed obvious cold injury symptoms. Their apical shoots including apical buds and immature leaves were dehydrated and yellowish with a loss of vitality. Moreover, the OE-3 and OE-5 lines showed normal foliage status as before (Figure 3B). The shoots of OE-1 plants were also affected by the low temperature but less damaged as compared to those of WT. The observation indicates that the high overexpression of AtCBF3 in cassava plants increases their tolerance to low-temperature stress in a dose-dependent manner.

AtCBF3 Overexpression Protects Cassava against Cold-Induced Cell Damage

Under the normal condition, the content of proline in seedlings of OE lines was similar to that of WT. A significant increase of proline content was detected in the OE lines than that of WT under -2°C stress (Figure 4A, upper panel) with a maximum of 52.9% increase at 12 h and 57% at 24 h. Strikingly, the MDA contents were always lower in OE lines than that in WT. Upon treatment, the minimum level of MDA in OE lines was about 26.9% that of WT; after 24 h treatment, the minimum level of MDA in OE lines only reached about 20.2% that of WT (Figure 4A, lower panel). Similarly, the electrolyte leakage in the leaf disks was also less in OE lines than WT: about half of WT after -5°C treatment (Figure 4B). These results indicate that the overexpression of AtCBF3 protects the cassava with less free radical production and from cellular membrane injury caused by cold stress.

FIGURE 4

FIGURE 4. Changes of proline content, MDA level, and electrolyte leakage in Arabidopsis CBF3 overexpressing cassava. (A) Proline concentration and MDA level in the WT and transgenic OE plants under cold treatment (-2°C) for 0, 12, and 24 h. FW, fresh weight. (B) Electrolyte leakage of WT and OE plants before and after cold treatment (-2 and -5°C) for 12 h. Data are presented as mean ± SD of three independent assays; asterisks indicate significant differences compared with the WT at ^∗∗P < 0.01 by Student’s t-test.

AtCBF3 Activates Many Stress-Related Genes in Transgenic Cassava

In order to speculate whether the AtCBF3 improves the abiotic stress tolerance of transgenic cassava through CBF cold-responsive pathway and the endogenous CBF-regulon response to stress, the transcript profiles of potential CBF-targeted genes (Table 1) were analyzed. These profiles were assessed using the apical bud, young leaf, mature leaf, old leaf, petiole, and young stem of field-grown WT and OE-3 plants under three different day/night temperatures (September 33°C/25°C as normal temperature, T1; October 25°C/16°C as cold treatment I, T2; November 15°C/8°C as cold treatment II, T3). The homologous genes in the cassava genome sequence from Phytozome¹ were discovered by protein sequence alignment with Arabidopsis stress-related genes. Several homologous genes, including MeRAP2.1, MeERD7, MeCOR47, MeKIN2, MeGolS3, MeLTI30, MeLEA14, MeOEP16, MeCOR78, and MeATDI21, were identified and their promoter regions ca. 2000 bp length were cloned. Six out of 10 genes belonged to the CBF-target genes as they harbored the CBF binding element DRE/CRT (G/ACCGAC, Yamaguchi-Shinozaki and Shinozaki, 1994; Stockinger et al., 1997). The other three genes, whose promoters did not contain this element, might be regulated by CBF3 via other cis-elements or other regulators mediated by CBF3. However, the promoter of MeCOR78, a COR gene, was not found (Table 1).

TABLE 1

TABLE 1. CBF3-targeted orthologous genes in cassava.

Compared to WT, all tissues of the OE-3 line detected an increased expression of CBF3, the highest in young leaf, petiole, and young stem, whereas the apical bud showed the lowest expression of the gene (Figure 5). Interestingly, all the stress-related genes exhibited little expression changes in the apical bud of both WT and OE-3 plants indicating an unknown upstream factor that might be inhibiting the expression induction of these stress-response genes in cassava meristematic tissues. In other tissues, most of the genes showed upregulated expression by the cold treatment I (T2) and maintained similar levels as WT by cold treatment II (T3) in the OE-3 line. Nevertheless, specific response of these genes to cold treatment varies among different tissues. For example, in the young and mature leaf of OE-3 line, the expression of MeRAP2.1, MeERD7, MeCOR47, and MeKIN2 was obviously induced by the cold treatment I (T2); however, the expression of these genes was less affected by the treatment in WT. The expression of MeGolS3 only changed in the young stem by T2. Several genes whose promoters lacked the DRE/CRT element, especially in MeERD7, MeLTI30, and MeCOR78 showed less difference in expression between the WT and OE-3 plants under the normal or cold treatments. The expression of other genes such as MeLEA14 and MeATDI21, containing the DRE/CRT element in their promoters was not affected by the overexpression of AtCBF3 (Figure 5), indicating the variations between Arabidopsis and cassava CBF regulons. Taken together, cassava CBF regulon differed from that of Arabidopsis and its regulation showed temporal and spatial patterns in response to heterologous CBF and cold treatment.

FIGURE 5

FIGURE 5. Expression patterns of Arabidopsis CBF3 and alleged members of CBF-regulon in cassava under the seasonal temperature changes. Six different tissues of WT and the OE-3 transgenic plants were used, including apical bud, young leaf, mature leaf, old leaf, petiole, and young stem. Description of cassava genes is listed in Table 1.

Since cold treatment promoted excessive proline accumulation in OE lines (Figure 4A), the expression profiles of P5CS (Δ1-pyrroline-5-carboxylate synthase) and ProDH (proline dehydrogenase), two key genes controlling the balance of biosynthetic and catabolic pathways (Sharma et al., 2011), were also checked in these different tissues. Upregulation of MeP5CS and downregulation of MeProDH were detected in the transgenic OE-3 line compared with those of WT under normal and cold treatment conditions (Figure 6). This suggested that ectopic overexpression of AtCBF3 might favor the modulation of stress-related gene expression in response to abiotic stress in transgenic cassava.

FIGURE 6

FIGURE 6. Expression patterns of MeP5CS and MeProDH in cassava under the seasonal temperature changes. Six different tissues of WT and the OE-3 transgenic plants were used, including apical bud, young leaf, mature leaf, old leaf, petiole, and young stem.

Constitutive Expression of AtCBF3 Enhances Cassava Tolerance to Drought

To examine the drought stress tolerance of OE transgenic cassava, the 3-month-old plants of OE lines and WT grown in pots were subjected to drought treatment by water depletion. Before drought stress, all the plants exhibited active growth status (Figure 7A). After water depletion for 3 weeks, WT plants showed distinct injury phenotype including severe wilting and dehydrated leaves. Contrastingly, the OE plants consisted of more green leaves and relatively less dehydration phenotype (Figure 7A), especially in OE-3 and OE-5 plants. Their RWC was dramatically higher than WT, about ninefold after the drought treatment, although no difference was recorded between the OE and WT plants under normal growth conditions (Figure 7B). Furthermore, the electrolyte leakage of leaves in OE plants was reduced significantly than that in WT under normal growth conditions. Under drought stress, WT showed approximately 90% electrolyte leakage, while that of OE lines was only 15–40% (Figure 7C). The phenotype indicates that the integrity of the membrane was dramatically injured in WT but preserved in OE lines. These results demonstrate that the overexpression of CBF3 resulted in improved drought stress tolerance in transgenic cassava plants.

FIGURE 7

FIGURE 7. Performance of Arabidopsis CBF3 overexpressing cassava in response to drought. (A) 3-month-old pot-grown plants in the greenhouse before and after drought treatment, i.e., withheld water for 3 weeks. (B,C) Relative water content (B) and electrolyte leakage (C) of the WT and OE lines under normal and drought stress. Data are presented as mean ± SD of three independent assays; asterisks indicate significant differences compared with the WT at ^∗∗P < 0.01 by Student’s t-test.

AtCBF3 Negatively Regulates Anthocyanin Biosynthesis in Transgenic Cassava

As illustrated in Figure 3B, the absence of anthocyanin accumulation was noticed in the OE plants, especially OE-3 and OE-5. In cassava TMS60444, apical buds, young leaves, and the base region of petioles show the purple color of anthocyanins. A quantitative analysis of anthocyanins from apical buds and young leaves demonstrated a significant decrease of anthocyanins in the OE lines (Figure 8A). In their top leaves, the expression level of several anthocyanin biosynthetic genes in cassava was substantially decreased, including 4CL, CHS, CHI, and ANS. In addition, only the BZ1 gene showed an upregulated expression, possibly due to the feedback regulation (Figure 8B). In response to the downregulated expression of these genes, the expression of several TFs, such as MYB, MYC, WD-40-like that have been reported to regulate anthocyanin biosynthesis (Petroni and Tonelli, 2011), was also analyzed. Among these TFs, MePAP2 and MeTTG1 showed about 50% reduction in their expression (Figure 8C), although their promoter regions did not harbor the DRE/CRT element (data not shown).

FIGURE 8

FIGURE 8. Characterization of anthocyanin biosynthesis in Arabidopsis CBF3 overexpressing cassava. (A) Changes of anthocyanin content in the apical buds, the third and fourth leaves from the top of WT and OE plants. (B) Expressional changes of genes related to anthocyanin biosynthesis. PAL, phenylalanine ammonia-lyase; 4CL, 4-coumarate-CoA ligase; C4H, trans-cinnamate-4-monooxygenase; CHS, naringenin-chalcone synthase; CHI, chalcone isomerase; F3H, naringenin 3-dioxygenase; F3′H, flavonoid 3′-hydroxylase; F3′5′H, flavonoid 3′,5′-hydroxylase; DFR, dihydroflavonol4-reductase; ANS, anthocyanidin synthase; BZ1, anthocyanidin 3-O-glucosyltransferase. (C) Expressional changes of cassava transcription factors (EGL1/GL3, PAP2, PAP1, MYB4, and TTG1) that regulate anthocyanin biosynthesis.

Discussion

The CBF regulatory pathway is well established as the core component of cold response controlling the expression of several COR genes in Arabidopsis and temperate crops (Chinnusamy et al., 2007; Zhao et al., 2016). In the present study, we generated transgenic cassava plants overexpressing Arabidopsis CBF3 gene and found that such plants showed improved stress tolerance to cold and drought (Figures 3 and 7). Consequently, the AtCBF3-overexpressing cassava, under normal and stress conditions, showed many physiological alterations such as improved accumulation of proline, reduced membrane injury, and increased RWC, compared to those of WT. Similar results have been reported in transgenic plants overexpressing Arabidopsis CBF3 (Nakashima et al., 2009). These results indicated that Arabidopsis CBF3 is functional and plays a similar role in enhancing tolerance to abiotic stress in the tropical species.

The expression of the corresponding CBF-regulon is essential for plants responding to various stresses and for the development of stress tolerance (Chinnusamy et al., 2004; Nakashima et al., 2009; Zhao et al., 2016). In Arabidopsis, CBF-regulon encompasses hundreds of genes that are involved in multiple functions, for example, RAP2.1, COR47, OEP16, Gols3, and LEA14. These members of CBF-regulon are stress-responsive and play vital roles in enhancing abiotic stress in Arabidopsis (Novillo et al., 2007; Park et al., 2015). CBF regulons are also species-specific and show variation between the temperate and tropical plants (Zhang et al., 2004; Carvallo et al., 2011). Although CBF-regulon has not yet been fully studied in cassava, among the 11 putative CBF-targeted genes identified in cassava genome (Table 1), most of them were highly inducible by the cold treatment in certain tissues, for example, leaves. Interestingly, the inducibility is significantly enhanced in the AtCBF3-overexpressing cassava for some genes, indicating that the CBF cold-responsive pathway is repressed up to some extent under cold stress and could be activated by AtCBF3. These CBF regulon members showed a strong temporal and spatial expression in cassava, out of which some were totally unresponsive to cold treatment, implying the difference between cassava and Arabidopsis CBF-regulons.

Our result of cold treatment in field-grown plants also revealed the response collapse of CBF regulons under the extended cold treatment (T3 treatment). Such treatment caused the cell damage in various tissues of cassava plants, leading to the reduced expression of CBF-targeted genes. Recently, we found that the retarded MeCBF1 expression in response to cold and attenuated CBF-regulon might be responsible for the chilling sensitivity of cassava (An et al., Unpublished data). It is also in agreement with our previous findings that the cassava apical bud is more sensitive to cold stress due to the CBF regulon (An et al., 2012). With the advances in the cassava genome sequencing (Wang et al., 2014; Bredeson et al., 2016), the screening of the key TFs that dominate the cold-response pathway in cassava is plausible using multiple approaches.

There are several obvious phenotypic changes in the AtCBF3-overexpressing cassava: one is the retarded plant growth with leaf curling, another is reduced anthocyanin accumulation, and the third is reduced storage biomass. The phenotypic changes may be caused by the altered expression of downstream COR genes or TFs. Several studies have reported that the overexpression of Arabidopsis CBFs might lead to retarded plant growth with leaf curling in Arabidopsis, tomato, tobacco, rice, and wheat under unstressed conditions. This phenomenon may be mediated through putative interaction with other TFs that have a growth suppressing role in order to disturb the regulation of leaf morphogenesis (Jaglo-Ottosen et al., 1998; Gilmour et al., 2000; Hsieh et al., 2002b; Kasuga et al., 2004; Pellegrineschi et al., 2004). When cassava overexpresses the native MeCBF1 gene, less effect on the leaf and plant growth is observed (An et al., Unpublished data), indicating that cassava evolved with the different regulatory mechanism of CBFs. The overexpression of AtCBF3 in cassava also influences the expression of other TFs that regulate anthocyanin biosynthesis (Figure 8C), leading to reduced anthocyanin accumulation. On the other hand, the ectopic expression of a peach CBF in apple can increase anthocyanin accumulation (Wisniewski et al., 2011). All these characteristics suggest that the regulation and function of CBF may differ from others among plant species.

In summary, the ectopic expression of Arabidopsis CBF3 in cassava improves the plant performance against cold and drought. This reflects the conserved function of CBF in plants. The altered expression profiles in response to cold showed that the CBF-regulons in WT and transgenic cassava were different. Dramatic changes in the plant phenotype and downregulation of anthocyanin biosynthesis indicate the specific interplay of CBF3 with other native TFs or pathways, proving the functional divergence of CBF gene in evolution.

Genbank Accession Numbers of Cassava Genes

JQ807797 (MeLTI30), JQ807798 (MeLEA14), JQ807799 (MeERD7), JQ807800 (MeFAD7), JQ807801 (MeCOR47), JQ807802 (MeGolS3), JQ807803 (MeOEP16), JQ807805 (MeKIN2), JQ807806 (MeCOR78), JQ807807 (MeRAP2.1), JQ807808 (MeATDI21), JQ807809 (MeP5CS), JQ807810 (MeProDH).

Author Contributions

DA performed most of the experiments and drafted the manuscript. QM produced transgenic cassava. WY, GL, and WZ conducted part of field experiments and analysis. PZ coordinated and designed the study and revised the article.

Funding

This research was supported by grants from the National Key Technology R&D Program of China (No. 2015BAD15B01), the National Natural Science Foundation of China (No. 31271775; 31571729), and the Earmarked Fund for China Agriculture Research System (No. CARS-12-shzp).

Supplementary Material

The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016.01866/full#supplementary-material

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Footnotes

1. ^ https://phytozome.jgi.doe.gov/Mesculenta

References

Ahuja, I., de Vos, R. C., Bones, A. M., and Hall, R. D. (2010). Plant molecular stress responses face climate change. Trends Plant Sci. 15, 664–674. doi: 10.1016/j.tplants.2010.08.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Alves, A. A. C. (2002). “Cassava botany and physiology,” in Cassava: Biology, Production and Utilization, eds R. J. Hillocks, J. M. Thresh, and A. C. Bellotti (Wallingford: CABI Publishing), 67–89.

Google Scholar

An, D., Yang, J., and Zhang, P. (2012). Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress. BMC Genomics 13:64. doi: 10.1186/1471-2164-13-64

PubMed Abstract | CrossRef Full Text | Google Scholar

Bates, L. S., Waldren, R. P., and Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207. doi: 10.1016/j.dental.2010.07.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Bohnert, H. J., Nelson, D. E., and Jensen, R. G. (1995). Adaptations to environmental stresses. Plant Cell 7, 1099–1111. doi: 10.1105/tpc.7.7.1099

PubMed Abstract | CrossRef Full Text | Google Scholar

Bredeson, J. V., Lyons, J. B., Prochnik, S. E., Wu, G. A., Ha, C. M., Edsinger-Gonzales, E., et al. (2016). Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity. Nat. Biotechnol. 34, 562–570. doi: 10.1038/nbt.3535

PubMed Abstract | CrossRef Full Text | Google Scholar

Carvallo, M. A., Pino, M. T., Jeknic, Z., Zou, C., Doherty, C. J., Shiu, S. H., et al. (2011). A comparison of the low temperature transcriptomes and CBF regulons of three plant species that differ in freezing tolerance: Solanum commersonii, Solanum tuberosum, and Arabidopsis thaliana. J. Exp. Bot. 62, 3807–3819. doi: 10.1093/jxb/err066

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, J. Q., Meng, X. P., Zhang, Y., Xia, M., and Wang, X. P. (2008). Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnol. Lett. 30, 2191–2198. doi: 10.1007/s10529-008-9811-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Chinnusamy, V., Schumaker, K., and Zhu, J. K. (2004). Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants. J. Exp. Bot. 55, 225–236. doi: 10.1093/jxb/erh005

PubMed Abstract | CrossRef Full Text | Google Scholar

Chinnusamy, V., Zhu, J., and Zhu, J. K. (2007). Cold stress regulation of gene expression in plants. Trends Plant Sci. 12, 444–451. doi: 10.1016/j.tplants.2007.07.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Cock, J. H. (1982). Cassava - a basic energy-source in the tropics. Science 218, 755–762. doi: 10.1126/science.7134971

PubMed Abstract | CrossRef Full Text | Google Scholar

Dubouzet, J. G., Sakuma, Y., Ito, Y., Kasuga, M., Dubouzet, E. G., Miura, S., et al. (2003). OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J. 33, 751–763. doi: 10.1046/j.1365-313X.2003.01661.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Fowler, S., and Thomashow, M. F. (2002). Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14, 1675–1690. doi: 10.1105/tpc.003483

PubMed Abstract | CrossRef Full Text | Google Scholar

Gaxiola, R. A., Li, J. S., Undurraga, S., Dang, L. M., Allen, G. J., Alper, S. L., et al. (2001). Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc. Natl. Acad. Sci. U.S.A. 98, 11444–11449. doi: 10.1073/pnas.191389398

PubMed Abstract | CrossRef Full Text | Google Scholar

Gilmour, S. J., Fowler, S. G., and Thomashow, M. F. (2004). Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities. Plant Mol. Biol. 54, 767–781. doi: 10.1023/B:PLAN.0000040902.06881.d4

PubMed Abstract | CrossRef Full Text | Google Scholar

Gilmour, S. J., Sebolt, A. M., Salazar, M. P., Everard, J. D., and Thomashow, M. F. (2000). Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiol. 124, 1854–1865. doi: 10.1104/pp.124.4.1854

PubMed Abstract | CrossRef Full Text | Google Scholar

Gilmour, S. J., Zarka, D. G., Stockinger, E. J., Salazar, M. P., Houghton, J. M., and Thomashow, M. F. (1998). Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J. 16, 433–442. doi: 10.1046/j.1365-313x.1998.00310.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Hillocks, R. J., Thresh, J. M., and Bellotti, A. (2002). Cassava: Biology, Production and Utilization. Wallingford: CABI.

Google Scholar

Hodges, D. M., DeLong, J. M., Forney, C. F., and Prange, R. K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207, 604–611. doi: 10.1007/s004250050524

CrossRef Full Text | Google Scholar

Howeler, R. H. (2015). “Sustainable cassava production in Asia for multiple uses and for multiple markets,” in Proceedings of the Ninth Regional Workshop held in Nanning, Guangxi

Google Scholar

Hsieh, T. H., Lee, J. T., Charng, Y. Y., and Chan, M. T. (2002a). Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. Plant Physiol. 130, 618–626. doi: 10.1104/pp.006783

PubMed Abstract | CrossRef Full Text | Google Scholar

Hsieh, T. H., Lee, J. T., Yang, P. T., Chiu, L. H., Charng, Y. Y., Wang, Y. C., et al. (2002b). Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiol. 129, 1086–1094. doi: 10.1104/pp.003442

PubMed Abstract | CrossRef Full Text | Google Scholar

Huang, L., Ye, Z., Bell, R. W., and Dell, B. (2005). Boron nutrition and chilling tolerance of warm climate crop species. Ann. Bot. 96, 755–767. doi: 10.1093/aob/mci228

PubMed Abstract | CrossRef Full Text | Google Scholar

Ito, Y., Katsura, K., Maruyama, K., Taji, T., Kobayashi, M., Seki, M., et al. (2006). Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice. Plant Cell Physiol. 47, 141–153. doi: 10.1093/pcp/pci230

PubMed Abstract | CrossRef Full Text | Google Scholar

Jaglo, K. R., Kleff, S., Amundsen, K. L., Zhang, X., Haake, V., Zhang, J. Z., et al. (2001). Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol. 127, 910–917. doi: 10.1104/pp.010548

PubMed Abstract | CrossRef Full Text | Google Scholar

Jaglo-Ottosen, K. R., Gilmour, S. J., Zarka, D. G., Schabenberger, O., and Thomashow, M. F. (1998). Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science 280, 104–106. doi: 10.1126/science.280.5360.104

PubMed Abstract | CrossRef Full Text | Google Scholar

Jansson, C., Westerbergh, A., Zhang, J., Hu, X., and Sun, C. (2009). Cassava, a potential biofuel crop in (the) people’s republic of China. Appl. Energy 86, S95–S99. doi: 10.1016/j.apenergy.2009.05.011

CrossRef Full Text | Google Scholar

Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., and Shinozaki, K. (1999). Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. Biotechnol. 17, 287–291. doi: 10.1038/7036

PubMed Abstract | CrossRef Full Text | Google Scholar

Kasuga, M., Miura, S., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2004). A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol. 45, 346–350. doi: 10.1093/pcp/pch037

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, J., Zheng, Q., Ma, Q., Gadidasu, K. K., and Zhang, P. (2011). Cassava genetic transformation and its application in breeding. J. Integr. Plant Biol. 53, 552–569. doi: 10.1111/j.1744-7909.2011.01048.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Mickelbart, M. V., Hasegawa, P. M., and Bailey-Serres, J. (2015). Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. Nat. Rev. Genet. 16, 237–251. doi: 10.1038/nrg3901

PubMed Abstract | CrossRef Full Text | Google Scholar

Mizoi, J., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2012). AP2/ERF family transcription factors in plant abiotic stress responses. Biochim. Biophys. Acta 1819, 86–96.

Google Scholar

Morran, S., Eini, O., Pyvovarenko, T., Parent, B., Singh, R., Ismagul, A., et al. (2011). Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors. Plant Biotechnol. J. 9, 230–249. doi: 10.1111/j.1467-7652.2010.00547.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Nakashima, K., Ito, Y., and Yamaguchi-Shinozaki, K. (2009). Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol. 149, 88–95. doi: 10.1104/pp.108.129791

PubMed Abstract | CrossRef Full Text | Google Scholar

Nguyen, T. L. T., Gheewala, S. H., and Garivait, S. (2007). Full chain energy analysis of fuel ethanol from cassava in Thailand. Environ. Sci. Technol. 41, 4135–4142. doi: 10.1021/es0620641

PubMed Abstract | CrossRef Full Text | Google Scholar

Novillo, F., Medina, J., and Salinas, J. (2007). Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proc. Natl. Acad. Sci. U.S.A. 104, 21002–21007. doi: 10.1073/pnas.0705639105

PubMed Abstract | CrossRef Full Text | Google Scholar

Park, S., Lee, C. M., Doherty, C. J., Gilmour, S. J., Kim, Y., and Thomashow, M. F. (2015). Regulation of the Arabidopsis CBF regulon by a complex low-temperature regulatory network. Plant J. 82, 193–207. doi: 10.1111/tpj.12796

PubMed Abstract | CrossRef Full Text | Google Scholar

Pellegrineschi, A., Reynolds, M., Pacheco, M., Brito, R. M., Almeraya, R., Yamaguchi-Shinozaki, K., et al. (2004). Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47, 493–500. doi: 10.1139/g03-140

PubMed Abstract | CrossRef Full Text | Google Scholar

Petroni, K., and Tonelli, C. (2011). Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci. 181, 219–229. doi: 10.1016/j.plantsci.2011.05.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Sharma, S., Villamor, J. G., and Verslues, P. E. (2011). Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential. Plant Physiol. 157, 292–304. doi: 10.1104/pp.111.183210

PubMed Abstract | CrossRef Full Text | Google Scholar

Shinozaki, K., Yamaguchi-Shinozaki, K., and Seki, M. (2003). Regulatory network of gene expression in the drought and cold stress responses. Curr. Opin. Plant Biol. 6, 410–417. doi: 10.1016/S1369-5266(03)00092-X

CrossRef Full Text | Google Scholar

Stockinger, E. J., Gilmour, S. J., and Thomashow, M. F. (1997). Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. U.S.A. 94, 1035–1040.

PubMed Abstract | Google Scholar

Thomashow, M. F. (1999). Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Phys. 50, 571–599. doi: 10.1146/annurev.arplant.50.1.571

PubMed Abstract | CrossRef Full Text | Google Scholar

Thomashow, M. F., Gilmour, S. J., Stockinger, E. J., Jaglo-Ottosen, K. R., and Zarka, D. G. (2001). Role of the Arabidopsis CBF transcriptional activators in cold acclimation. Physiol. Plant. 112, 171–175. doi: 10.1034/j.1399-3054.2001.1120204.x

CrossRef Full Text | Google Scholar

Wang, W., Feng, B., Xiao, J., Xia, Z., Zhou, X., Li, P., et al. (2014). Cassava genome from a wild ancestor to cultivated varieties. Nat. Commun. 5:5110. doi: 10.1038/ncomms6110

PubMed Abstract | CrossRef Full Text | Google Scholar

Wisniewski, M., Norelli, J., Bassett, C., Artlip, T., and Macarisin, D. (2011). Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus × domestica) results in short-day induced dormancy and increased cold hardiness. Planta 233, 971–983. doi: 10.1007/s00425-011-1358-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Xu, J., Yang, J., Duan, X., Jiang, Y., and Zhang, P. (2014). Increased expression of native cytosolic Cu/Zn superoxide dismutase and ascorbate peroxidase improves tolerance to oxidative and chilling stresses in cassava (Manihot esculenta Crantz). BMC Plant Biol. 14:208. doi: 10.1186/s12870-014-0208-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Yamaguchi-Shinozaki, K., and Shinozaki, K. (1994). A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6, 251–264. doi: 10.2307/3869643

CrossRef Full Text | Google Scholar

Zhang, P., and Gruissem, W. (2004). “Production of transgenic cassava (Manihot esculenta Crantz),” in Transgenic Crops of the World – Essential Protocols, ed. I. S. Curtis (Dordrecht: Kluwer Academic Publishers), 301–319.

Google Scholar

Zhang, X., Fowler, S. G., Cheng, H., Lou, Y., Rhee, S. Y., Stockinger, E. J., et al. (2004). Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis. Plant J. 39, 905–919. doi: 10.1111/j.1365-313X.2004.02176.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, C., Zhang, Z., Xie, S., Si, T., Li, Y., and Zhu, J. K. (2016). Mutational evidence for the critical role of CBF transcription factors in cold acclimation in Arabidopsis. Plant Physiol. 171, 2744–2759. doi: 10.1104/pp.16.00533

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, J., Dong, C. H., and Zhu, J. K. (2007). Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Curr. Opin. Plant Biol. 10, 290–295. doi: 10.1016/j.pbi.2007.04.010

PubMed Abstract | CrossRef Full Text | Google Scholar