Inclusivity Essentials_ Creating Accessible Websites for Nonprofits .pdf
Sharing session exp 4: AAS Spectroscopy
1. BY:
NUR SHUHADA NABILLA
NURUL AFIFA
NUR LAILI
NUR ATIELIA
RAIHAN IZZATI
EXPERIMENT 4
FLAME ATOMIC ABSORPTION
SPECTROSCOPY (AAS)
2. Objectives:
To learn how to operate a flame AAS instrument.
To plot a standard calibration curve for
determination of Ca in a sample.
To determine the amount of Ca in a sample using
the standard calibration curve.
6. Preparation of standard solutions
A 5.00mL of 1000ppm stock
solution was pipetted and
transferred to 50.0mL
volumetric flask.
The solution was diluted
with HNO3 (1%) until the
mark. The solution was
labeled as ‘100ppm’.
7. Using the 100ppm solution and a 50mL volumetric flask,
a series of standard solutions was prepared by having
the following concentrations:
1ppm, 2ppm, 3ppm, 4ppm, 5ppm
And unknown solution was obtained.
The absorbance of the standard solutions in (2) was
measured followed by the unknown.