Heather Watson's French Open ended in the second round for the fifth time as she came off second best against former champion Svetlana Kuznetsova.
It was a tough draw for Watson against a player who won the title in 2009 and has reached at least the quarter-finals on six other occasions.
Watson competed well in the second set but her serve was vulnerable throughout and she went down 6-1 6-3 in and hour and 12 minutes.
The British number two did not hold her serve for the first 48 minutes of the match, losing her first five service games.
Kuznetsova was much the stronger in the opening set and Watson bounced her racket angrily on the ground in the third game as she struggled to gain a foothold.
The second set was much more competitive, with Watson finally holding serve to lead 2-1, but she then lost four games in a row.
She had her chances, particularly in a lengthy seventh game, but the power of Kuznetsova ultimately told.
Watson has made the second round at Roland Garros in five of the last six years but has not been able to take the next step.
The 24-year-old's exit marked the end of British interest in the women's singles, with Johanna Konta and Laura Robson both having lost in the first round.
Former finalist Simona Halep survived a first-set wobble to reach the third round for only the second time in her career with a 7-6(5) 6-2 win over Kazakhstan's Zarina Diyas.
The Romanian sixth seed surrendered her serve twice in the opening four games to trail 4-1 but delved into her wealth of experience to finally subdue her 90th-ranked opponent 7-5 in the tiebreak.
More exchanges of breaks followed in the second set before Halep finally found her groove to take a 5-2 lead and set up three match points on Diyas' serve.
The first one went begging but Halep's gritty baseline play paid off in the next point as Diyas bowed out after flicking a forehand long to end a 10-shot rally.
"The first set was very tough and I was very nervous as I seemed to be fighting for every point," Halep said in a courtside interview.
The good news for Halep is that the only previous time she reached the last 32, she made it all the way to the 2014 Roland Garros final.
Halep and her bizarre black and white zebra print dress will be back on court on Friday when she takes on Japan's Naomi Osaka for a place in the fourth round.
With three of the top seven seeds out of the women's draw in the first round at the French Open, there was no guarantee Garbine Muguruza would stroll into the third round at Roland Garros.
The Spanish fourth seed, however, barely broke sweat as she swept aside French wildcard Myrtille Georges 6-2 6-0 on a sun-bathed Court Philippe Chatrier.
On Monday, third seed Angelique Kerber, fifth seed Victoria Azarenka and seventh seed Roberta Vinci were knocked out in the first round.
Second seed Agnieszka Radwanska took advantage of Caroline Garcia's weak serve to advance into the third round with a 6-2 6-4 victory.
The Pole, who will next face Czech 30th seed Barbora Strycova, broke six times as her French opponent looked nothing like the player who won the Strasbourg International last Saturday.
Garcia won a higher percentage of points on her second than on her first serve, which was all too easy to read for Radwanska, who reached the quarter-finals at Roland Garros in 2013.
Garcia threatened a comeback in the second set after falling 3-0 down, but two successive double faults handed Radwanska her third match point, which she converted as the Frenchwoman mishit a backhand.
