Emma Pooley will race alongside world champion Lizzie Armitstead in the Great Britain team at the Women's Tour de Yorkshire on April 30.
Pooley retired from professional cycling following the 2014 Commonwealth Games to turn her attentions to long-distance triathlon and duathlon. She won the long-distance duathlon (cycling and running) world titles in 2014 and 2015.
She has been tempted to return to cycling by the undulating time-trial course for the Rio Olympics, where she is also expected to be selected in support of Armitstead for the road race.
The 33-year-old, who won Olympic time-trial silver in Beijing in 2008, will line up alongside Armitstead for the 135-kilometres race, which begins in Otley and finishes in Doncaster.
She said: "I'm really looking forward to joining up with my Great Britain team-mates for the Women's Tour de Yorkshire race and I'm looking forward to racing on the road again."
British Cycling technical director Shane Sutton said: "Emma is a phenomenal athlete and will be an asset to the team. Emma has committed herself to training for the Olympic time-trial event in Rio, and we appreciate and support the sacrifice she is making to be in the best possible shape for this event."