Issue 17, 2014
From the journal:

Analyst

Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides

Palaniappan Subramanian,a   Ievgen Mazurenko,b   Vladimir Zaitsev,bc   Yannick Coffinier,a   Rabah Boukherrouba  and  Sabine Szunerits*a  

* Corresponding authors

a Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France
E-mail: Sabine.Szunerits@iri.univ-lille1.fr

b Department of Analytical Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska str., 62a, 01033 Kyiv, Ukraine

c Chemistry Department, Pontifical Catholic University of Rio de Janeiro, 225 Rua Marquês de São Vicente, 22451, Rio de Janeiro, Brazil

Abstract

Coating boron-doped diamond nanowires (BDD NWs) with a conducting polymer, poly[3-(pyrrolyl)carboxylic acid], has been reported. Polymer coating was achieved through electropolymerization of 3-(pyrrolyl)carboxylic acid at the electrode interface by amperometrically biasing the BDD NWs interface until a predefined charge has passed. The poly[3-(pyrrolyl)carboxylic acid] modified BDD NWs (PPA–BDD NWs) were characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV). Using a deposition charge of 11 mC cm−2 resulted in a thin polymer film deposition. The availability of the carboxylic groups of the polymer coated BDD NWs electrode was demonstrated through copper ion (Cu2+) chelation. The resulting complex was successfully used for the site-specific immobilization of histidine-tagged peptides. The binding process was followed by electrochemical impedance spectroscopy (EIS). The Cu2+-chelated PPA–BDD NWs interface showed peptide loading capability comparable to those of commercially available interfaces and can be easily regenerated several times using ethylenediaminetetraacetic acid (EDTA).

Graphical abstract: Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides

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Article information

DOI
https://doi.org/10.1039/C4AN00146J
Article type
Paper
Submitted
20 Jan 2014
Accepted
10 Jun 2014
First published
11 Jun 2014

Analyst, 2014,139, 4343-4349

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Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides

P. Subramanian, I. Mazurenko, V. Zaitsev, Y. Coffinier, R. Boukherroub and S. Szunerits, Analyst, 2014, 139, 4343 DOI: 10.1039/C4AN00146J

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